The COVID-19 pandemic and the resultant lockdown has caused massive economic disruption, leading businesses to make a rapid transition and take a new approach to business strategy. Therefore, the aim of the paper is to examine if there are statistically significant gender differences in work satisfaction, work engagement, and work efficiency among employees who work from home during the COVID-19 pandemic. Further, the paper aims to identify the importance of individual dimensions of work satisfaction, work engagement, and work efficiency, and gender differences perspective. The research is based on a survey of 785 employees in Slovenian companies. The factor analysis and the t-test for two independent samples were used to test the research hypotheses. Findings: The results show significant gender differences in work satisfaction, work engagement, and work efficiency among employees who work from home during the COVID-19 pandemic. The paper provides change management insights and recommendations to assist companies in minimizing the negative impact of the COVID-19 pandemic on their employees.