The fabrication of novel thin-film nanocomposite (TFN) membranes with superior flux, rejection as well as prolonged stability is being requested by membrane industries for saving energy and cost. In this regard, in the present research work we developed novel TFN membranes using amine-rich synthetic talc (NHST) nanosheets within diethylenetriamine (DETA) and trimesoyl chloride (TMC) monomers over a polydopamine (PDA) coating layer. Different concentrations (0.2–1 %) of NHST nanosheets are applied for the fabrication of TFN membranes and comparatively examined with a thin-film composite (TFC) membrane. In-situ synthesized NHST nanosheet phases are confirmed by several characterization techniques and results are explored in depth. All prepared membranes are well investigated to confirm the incorporation of NHST by several characterization techniques, like FE-SEM, ATR-FTIR, HR-TEM, XPS, PXRD, TGA, and contact angle. It is found that a small amount of NHST doping in the TFN layer considerably improved the performance in the form of permeability and rejection. The developed TFN membranes showed hydrophilic characteristics as confirmed by the contact angle. Water flux performance of TFN membranes was also observed comparatively higher than TFC membranes. Optimized NHST-PDT-TFN2 membrane with NHST concentration 0.5 % resulted in 98.96, 95.35, 93.83 and 21.66 % rejection for Na2SO4, MgSO4, CuSO4 and NaCl salts, respectively together with permeance up to 24.45, 22.72, 26.45 and 30.75 Lm−2h−1b−1. The present research finding concludes that the incorporation of a little quantity of NHST nanosheets in the TFN layer plays a unique potential role in the enhancement of flux and rejection of targeted mineral salts. Additionally, the NHST-PDT-TFN membranes revealed outstanding antibacterial properties signifying that contact with microorganisms does not affect the performance and sustains the fouling resistance. The antifouling study of the membranes concluded that the addition of nanomaterials has led to enhanced antifouling tendency and low irreversible fouling, improving the longevity of the membranes. Display omitted •Aminofunctionlized synthetic talc nanosheets are used first time as TFN nanofillers.•PDA-coated TFC and TFN membranes were prepared by interfacial polymerization method.•The resultant novel NHST-PDT-TFN2 membrane exhibited the highest performance.•Prepared TFN membranes show excellent antifouling and antibacterial properties.