Abstract The objectives were to examine effects of litter size on insulin sensitivity in multiparous sows at the end of pregnancy. Twelve sows were allocated in two treatments after weaning: control (CTR) or ligature of the left oviduct (LIG). At 68 d of the subsequent pregnancy, catheters were implanted in a jugular vein, in a carotid artery, and in the main vein draining one uterine horn. A blood flow probe was fitted around the artery irrigating the same uterine horn. A meal test, a tolerance test, and an euglycemic hyperinsulinemic clamp test were performed at 108 ± 3 d of pregnancy. Serial blood samples were drawn simultaneously from the uterine vein and the carotid artery before and during the tests. The number of fetuses in the studied uterine horn was lower (3.7 vs. 8.0, P < 0.001), and piglets at birth were heavier (1.71 vs. 1.31 kg, P = 0.04) in the LIG sows than in the CTR sows. Treatment did not affect uterine blood flow (UBF), but UBF/fetus in the uterine horn was greater for the LIG treatment (0.67 vs. 0.34 L/min, P = 0.002). During meal test, glycemia, glucose uptake in the uterine horn and glucose uterine uptake/fetus were similar in both groups of sows, while insulin levels were higher in the LIG sows (P = 0.04). The decrease of NEFA concentrations was similar across treatments. Glucose half-life did not differ between treatments (13.4 min as a mean; P = 0.63) during tolerance test, but area under the insulin curve was greater in the LIG sows (P = 0.02). The glucose infusion rate during euglycemic hyperinsulinemic clamps was lower in the LIG sows than in CTR sows (6.1 ± 0.2 vs. 7.8 ± 0.1 mg glucose.kg−1 min−1; P = 0.01). The LIG sows are less sensitive to insulin than the CTR sows without adjustment of maternal glycemia and glucose tolerance. Insulin sensitivity adaptation to litter size in late pregnancy of sows would rather be connected to growth rate than to number of fetuses.