Display omitted •The silver metallic nanoparticles can be synthesized successfully using Cotinus coggygria leaf extract as a reducing agent and a standard solution of AgNO3 as a precursor.•As per the detailed Scopus survey, this is the first work documenting the greener production of silver nanoparticles using Cotinus coggygria leaves extract.•The green synthesized particles are crystalline, cylindrical with an average size of 27 nm.•The synthesized silver nanoparticles showed antibacterial activities against microorganisms like Staphylococcus aureus and Escherichia coli.•The antifungal analysis was also performed against Salmonella typhii and found that they can inhibit fungal activity quickly.•The silver nanoparticles synthesized can be a better option for controlling the growth of mosquitos and has much relevance in public health protection. Green synthesis of nanoparticles is comparatively cheaper, eco-friendly, and helps to analyze the anti-microbial nature of the synthesized particles. Through this study, the silver nanoparticles can be synthesized successfully using Cotinus coggygria leaf extract as a reducing agent and a standard solution of AgNO3 as a precursor. A strong peak at 450–480 nm in the UV–Visible spectra revealed that silver nanoparticles (AgNPs) were synthesized using green methods. The synthesized particles were further characterized using XRD, SEM, and EDS. XRD results confirmed that the green synthesized particles are highly crystalline, with an average size of 27 nm. From scanning electron microscopy, it is found that the particles are cylindrical in shape. With an inhibitory zone of 7 mm for Staphylococcus aureus and 9 mm for Escherichia coli, respectively, the synthesized silver nanoparticles demonstrated antibacterial activity against these pathogens. The anti-fungal analysis was also performed against Salmonella typhi and found that they can inhibit fungal activity with an inhibition zone of 22 mm. Furthermore, the larvicidal activity of the synthesized nanoparticles was explored following the bioassays performed by following the WHO guidelines. The silver nanoparticles synthesized can be a better option for controlling the growth of mosquitos and has much relevance in public health protection. As the concentration of synthesized AgNPs increases, larval mortality is also higher, even with the shorter exposure period. Our research revealed that Cotinus coggygria leaf extracts have good capping and reducing agents for producing stable AgNPs. Here, a one-step, quick, environmentally friendly, non-toxic approach is used instead of traditional physical and chemical techniques. This study has important medical implications and could pave the way for future research.