Background and Objectives: Urinary tract infections occur through the colonization of microbes in the urinary tract. One of the major challenges in the antibacterial treatment of these infections is an increase in antibiotic resistance. The aim of this study was to determine the prevalence of bacterial agents causing urinary infections and its antibiotic resistance patterns in patients referred to Shohada hospital in Khorramabad. Materials and Methods: In this cross-sectional study, 740 bacterial isolates were isolated and detected from urinary culture in Shohada Hospital of Khorramabad during one year (2016-2017). Antibiotic sensitivity pattern was measured by disc diffusion method. Results were analyzed using STATA-12 software. Results: The highest isolate was Escherichia coli (61.43%) and the least frequent was related to Citrobacer (0.27%). Isolated Escherichia coli had most resistance to nalidixic acid (54.18%). Isolates that picked up in different parts of the hospital showed the highest resistance to cotrimoxazole. Conclusions: Antibiotic resistance is high in isolates that picked up from urine culture of patients. Since, distribution of antibiotic resistance is variable in different regions and periods of time, periodic monitoring of antibiotic resistance is recommended to control the infection.