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Sun, Tieqiang; Xu, Zehua; Yuan, Shuai; Liu, Xiao; Chen, Zongfen; Han, Zhenyu; Liu, Wentao; Fan, Longxing; Yang, Han; Qie, Zhiwei; Ning, Baoan
Mikrochimica acta (1966), 04/2022, Letnik: 189, Številka: 4Journal Article
A method is described to achieve accurate quantitative detection of atrazine (ATZ) in maize by using lateral flow strips based on gold nanoparticles (GNPs) and a handheld scanning reader. GNPs of 15 nm in diameter were applied as label, and a lateral flow immune assay strip was prepared. The linear range was 5.01–95.86 ng mL −1 with a detection limit of 4.92 ng mL −1 in phosphate buffer, 4 times better than the readout by the naked eye. ATZ-spiked corn samples were also analysed. The accuracy of results of spiked samples was confirmed by ELISA and liquid chromatography-tandem mass spectrometry (HPLC), which proved the reliability of the proposed method. A handhold device with an optical scanning system was designed for on-site quantitative detection. Combined with the pretreatment, the assay could be completed in less than 20 min. Graphical abstract
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