The complex chemical characteristics of leaves of the representative species Globularia punctata were studied. The plant leaves contained large amounts of phenolic compounds (31% of the total analyzed constituents) followed by water-soluble proteins, total lipids, amino acids (AAs), and water-soluble sugars. A total of 22 AAs and two amides were identified with asparagine, glutamine, and proline dominating. An analysis of the lipids showed high concentrations of mono- and digalactosyldiacylglycerols and phosphatidylcholine. The fatty acids were dominated by dienoic and trienoic acids. The main source of linolenic acid was the glycolipid fraction, especially the MGDG and DGDG classes. The phospholipid fraction was more enriched in oleic and linoleic acids. The neutral lipid fraction contained various amounts of linoleic and linolenic acids.