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Otto, Oliver; Rosendahl, Philipp; Mietke, Alexander; Golfier, Stefan; Herold, Christoph; Klaue, Daniel; Girardo, Salvatore; Pagliara, Stefano; Ekpenyong, Andrew; Jacobi, Angela; Wobus, Manja; Töpfner, Nicole; Keyser, Ulrich F; Mansfeld, Jörg; Fischer-Friedrich, Elisabeth; Guck, Jochen
Nature methods, 03/2015, Letnik: 12, Številka: 3Journal Article
We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.
