...researchers have aimed to generate canker‐resistant citrus varieties by mutation of the EBE or coding region of the LOB1 gene using the CRISPR technology. LB and RB, the left and right borders of the T‐DNA region; GFP, green fluorescent protein; CaMV 35S and 35T, the cauliflower mosaic virus 35S promoter and terminator; NosP and NosT, the nopaline synthase gene promoter and terminator; NLS, nuclear localization signal; AtU3b, Arabidopsis U3b promoter; NptII, neomycin phosphotransferase II. c. The GFP‐p1380N‐SpCas9p:PumLOBP‐transformed Pummelo were GFP positive. d. Sequencing analyses of GFP‐p1380N‐SpCas9p:PumLOBP‐transformed Pummelo. Since only the Type II CsLOBP is present in Pummelo, a single sgRNA was designed to target both alleles. The sequencing results indicated that their unique chromatograms were same as one another for #Pum1 or #Pum6. ...the results confirmed that #Pum1 was homozygous and #Pum6 was biallelic.