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Li, Changyan; Li, Wei; Zhou, Zaihui; Chen, Hao; Xie, Conghua; Lin, Yongjun
Plant biotechnology journal, February 2020, Letnik: 18, Številka: 2Journal Article
...due to the unique function and disease resistance mechanism of the xa13, this gene is increasingly favoured in practical breeding (Hajira et al., ). ...we evaluated editing efficiency through the website (http://crispr.hzau.edu.cn/cgi-bin/CRISPR/CRISPR) and designed two gRNAs targeting the promoter of the xa13 gene (Figure a,c). ...the deletion of this promoter sequence would cause the Xa13 gene to lose its ability to be induced by bacterial blight, thus making the rice lacking this fragment resistant to bacterial blight (Figure e,f). Since the Xa13 gene plays a key role in pollen development, only a partial sequence of the Xa13 gene promoter was edited to remove its ability to induce expression without affecting gene expression and function in the anthers (Figure g). ...this deletion strategy can improve rice disease resistance without affecting rice fertility.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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