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  • Two-step strategy for the i...
    Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69-V70, France, August to December 2020
    Bal, Antonin; Destras, Gregory; Gaymard, Alexandre; Stefic, Karl; Marlet, Julien; Eymieux, Sébastien; Regue, Hadrien; Semanas, Quentin; d'Aubarede, Constance; Billaud, Geneviève; Laurent, Frédéric; Gonzalez, Claudia; Mekki, Yahia; Valette, Martine; Bouscambert, Maude; Gaudy-Graffin, Catherine; Lina, Bruno; Morfin, Florence; Josset, Laurence

    Euro surveillance : bulletin européen sur les maladies transmissibles, 01/2021, Letnik: 26, Številka: 3
    Journal Article

    We report the strategy leading to the first detection of variant of concern 202012/01 (VOC) in France (21 December 2020). First, the spike (S) deletion H69-V70 (ΔH69/ΔV70), identified in certain SARS-CoV-2 variants including VOC, is screened for. This deletion is associated with a S-gene target failure (SGTF) in the three-target RT-PCR assay (TaqPath kit). Subsequently, SGTF samples are whole genome sequenced. This approach revealed mutations co-occurring with ΔH69/ΔV70 including S:N501Y in the VOC.

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