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  • Pelagibius marinus sp. nov....
    Yang, Chen; Zhu, Suting; Sun, Chaoling; Ding, Lijian; Jung, Dawoon; He, Shan; Wang, Ning; Zhang, Weiyan

    International journal of systematic and evolutionary microbiology 72, Številka: 4
    Journal Article

    A Gram-stain-negative, rod-shaped, motile and aerobic marine bacterium, designated strain NBU2595 , was isolated from marine sediment sampled on Meishan Island, located in the East China Sea. Strain NBU2595 grew at 10-40 °C (optimum, 37 °C), at NaCl concentration of 0-10.0 % (w/v; optimum, 0.5 %) and at pH 6.0-8.0 (optimum, pH 7.0). Catalase and oxidase activities and H S production were positive. Methyl red reaction and hydrolysis of casein, starch and Tweens 20, 40, 60 and 80 were negative. The major cellular fatty acids were summed feature 8 (C 7 and/or C 6 ), C 2-OH and C 3-OH. The sole respiratory quinone was ubiquinone 10. The major polar lipids were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylmonomethylethanolamine. Comparative analysis of 16S rRNA gene sequences showed highest similarity to CL-UU02 (97.9%), and low similarities (<92.9 %) to other species. Phylogenetic analyses indicated that strain NBU2595 clustered with the genus and was closely related to CL-UU02 . The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU2595 and the related species of the genus were well below the thresholds for prokaryotic species delineation. The DNA G+C content was 66.5 mol%. Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU2595 should be placed in the genus as representing a novel species, for which the name sp. nov. is proposed. The type strain is NBU2595 (=MCCC 1K04773 =KCTC 82223 ).