Building on previous research demonstrating that demographic growth of racial minorities increases realistic threat and prejudice among majority group members, we examined whether demographic increases of groups associated with symbolic threat (lesbian, gay, bisexual, and transgender LGBT and nonreligious groups) increased realistic threat, symbolic threat, and/or prejudice. In a single-paper meta-analysis across four studies, participants who read that LGBT groups were becoming more prevalent in the United States exhibited heightened perceptions of realistic threat and (especially) symbolic threat from these groups, which in turn predicted anti-LGBT prejudice. Two similar studies examining the growth of nonreligious groups demonstrated weaker effects. Implications for America’s growing diversity and future directions for studying these effects are discussed.