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Hatsugai, Noriyuki; Katagiri, Fumiaki
Bio-protocol, 03/2018, Letnik: 8, Številka: 5Journal Article
We describe a protocol to measure the electrolyte leakage from plant tissues, resulting from loss of cell membrane integrity, which is a common definition of cell death. This simple protocol is designed to measure the electrolyte leakage from a tissue sample over a time course, so that the extent of cell death in the tissue can be monitored dynamically. In addition, it is easy to handle many tissue samples in parallel, which allows a high level of biological replication. Although the protocol is exemplified by cell death in in response to pathogen challenge, it is easily applicable to other types of plant cell death.
