E-viri
Recenzirano
-
Maeda, Hatsuo; Yamamoto, Kayoko; Nomura, Yoko; Kohno, Iho; Hafsi, Leila; Ueda, Noritsugu; Yoshida, Shoko; Fukuda, Masako; Fukuyasu, Yuka; Yamauchi, Yuji; Itoh, Norio
Journal of the American Chemical Society, 01/2005, Letnik: 127, Številka: 1Journal Article
Fluorometric detection of O2 -• is performed based on desulfonylation of 3 to the corresponding fluoresceins 4 through nucleophilic substitution, and this fluorescing process is quite specific toward O2 -• over H2O2, t-BuOOH, NaOCl, 1O2, HO•, NO•, and ONOO-. Furthermore, effects of glutathione, cytochrome P450 reductase/NADPH, and diaphorase/NADH are relatively small on the fluorescing process of probe 3 with X = Y = F, which is useful to detect O2 -• released from neutrophils stimulated by phorbol myristate acetate with satisfactory sensitivity.
