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Cao, Yunrui; Song, Yu; Fan, Xiaowei; Ma, Lei; Feng, Tingyu; Zeng, Junpeng; Xue, Changhu; Xu, Jie
Talanta (Oxford), 08/2024, Letnik: 276Journal Article
Hypoxanthine is a promising index for evaluating the freshness of various aquatic products. Combined the hydrogels containing upconversion nanoparticles (UCNPs), Co3O4 NPs, and N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt/4-amino-antipyrine (TOPS/4-AAP) with a smartphone, a portable sensor was developed for the convenient, sensitive detection of hypoxanthine. With the H2O2 from xanthine oxidase (XOD)-catalyzed reactions of hypoxanthine, the fluorescence of UCNPs was effectively quenched by the purple product produced from the oxidization of TOPS/4-AAP catalyzed by Co3O4 NPs exhibiting peroxidase activity, among which the color change could be transformed into digital signals for quantification of hypoxanthine. The Green value in the RGB analysis of the fluorescence image was negatively proportional to hypoxanthine concentration in the range of 2.5–20 mg/L with a detection limit of 0.69 mg/L and a quantitation limit of 2.30 mg/L. Finally, this sensor was applied for hypoxanthine detection in real aquatic products, showing potential application for freshness evaluation of aquatic products. Display omitted •UCNPs-Co3O4 NPs-TOPS/4-AAP-XOD nanosystem was developed for hypoxanthine detection.•Purple product catalyzed by Co3O4 NP quenched UCNPs' fluorescence through the IFE.•Agarose hydrogel and smartphones were adopted to improve stability and portability.•This method realized portable detection of hypoxanthine in real aquatic products.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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