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Pala, Laura; Mannucci, Edoardo; Pezzatini, Anna; Ciani, Silvia; Sardi, Jacopo; Raimondi, Laura; Ognibene, Agostino; Cappadona, Angela; Vannelli, Barbara G; Rotella, Carlo M
Biochemical and biophysical research communications, 10/2003, Letnik: 310, Številka: 1Journal Article
Glucagon-like peptide-1 (GLP-1), a meal-stimulated gastrointestinal insulinotropic hormone inactivated by dipeptidyl peptidase-IV (DPP-IV), is reduced in type 2 diabetic patients. The present study shows that 2-week exposure of human glomerular endothelial cells to high glucose (22 mM) determines a highly significant increase in DPP-IV activity and mRNA expression, which cannot be entirely accounted for by hyperosmolarity. On the other hand, incubation of purified DPP-IV in a buffer solution added with high glucose does not affect enzyme activity. These results suggest that high glucose increases expression and activity of DPP-IV, possibly contributing to GLP-1 reduction in type 2 diabetic patients.
