E-viri
Recenzirano
Odprti dostop
-
Yang, Ying; Stopka, Tomáš; Golestaneh, Nady; Wang, Yan; Wu, Kongming; Li, Anping; Chauhan, Bharesh K; Gao, Chun Y; Cveklová, Květa; Duncan, Melinda K; Pestell, Richard G; Chepelinsky, Ana B; Skoultchi, Arthur I; Cvekl, Aleš
EMBO journal, May 17, 2006, Letnik: 25, Številka: 10Journal Article
Pax6 and c‐Maf regulate multiple stages of mammalian lens development. Here, we identified novel distal control regions (DCRs) of the αA‐crystallin gene, a marker of lens fiber cell differentiation induced by FGF‐signaling. DCR1 stimulated reporter gene expression in primary lens explants treated with FGF2 linking FGF‐signaling with αA‐crystallin synthesis. A DCR1/αA‐crystallin promoter (including DCR2) coupled with EGFP virtually recapitulated the expression pattern of αA‐crystallin in lens epithelium and fibers. In contrast, the DCR3/αA/EGFP reporter was expressed only in ‘late’ lens fibers. Chromatin immunoprecipitations showed binding of Pax6 to DCR1 and the αA‐crystallin promoter in lens chromatin and demonstrated that high levels of αA‐crystallin expression correlate with increased binding of c‐Maf and CREB to the promoter and of CREB to DCR3, a broad domain of histone H3K9‐hyperacetylation extending from DCR1 to DCR3, and increased abundance of chromatin remodeling enzymes Brg1 and Snf2h at the αA‐crystallin locus. Our data demonstrate a novel mechanism of Pax6, c‐Maf and CREB function, through regulation of chromatin‐remodeling enzymes, and suggest a multistage model for the activation of αA‐crystallin during lens differentiation.
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.