Lectins possess unique binding properties and are of particular value in molecular recognition. However, lectins suffer from several disadvantages, such as being hard to prepare and showing poor storage stability. Boronate‐affinity glycan‐oriented surface imprinting was developed as a new strategy for the preparation of lectin‐like molecularly imprinted polymers (MIPs). The prepared MIPs could specifically recognize an intact glycoprotein and its characteristic fragments, even within a complex sample matrix. Glycan‐imprinted MIPs could thus prove to be powerful tools for important applications such as proteomics, glycomics, and diagnostics. Sweet‐test thing: Boronate‐affinity glycan‐oriented surface imprinting was used to prepare molecularly imprinted polymers (MIPs) with lectin‐like binding properties. As a proof of principle, glycan‐imprinted magnetic nanoparticles (MNPs) that enable the recognition of an intact glycoprotein and its characteristic fragments through binding to the attached glycans were prepared.