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Basu, Anirban; Bhattacharya, Subhash Chandra; Kumar, Gopinatha Suresh
International journal of biological macromolecules, February 2018, 2018-Feb, 2018-02-00, 20180201, Letnik: 107, Številka: Pt BJournal Article
•1-butyl-3-methylimidazolium bromide inhibits amyloid fibrillation in lysozyme.•Thioflavin T assay showed that fibrillation was suppressed.•Changes in the surface hydrophobicity of lysozyme were arrested in the presence of the ionic liquid.•AFM imaging showed that quantity of fibrils formed was reduced by the ionic liquid. Many proteins can abnormally fold to form pathological amyloid deposits/aggregates that are responsible for various degenerative disorders called amyloidosis. Here we have examined the anti-amyloidogenic potency of an ionic liquid, 1-butyl-3-methylimidazolium bromide, using lysozyme as a model system. Thioflavin T fluorescence assay demonstrated that the ionic liquid suppressed the formation of lysozyme fibrils significantly. This observation was further confirmed by the Congo red assay. Fluorescence microscopy, intrinsic fluorescence studies, nile red fluorescence assay, ANS binding assay and circular dichroism studies also testified diminishing of the fibrillogenesis in the presence of ionic liquid. Formation of amyloid fibrils was also characterized by α to β conformational transition. From far-UV circular dichroism studies it was observed that the β-sheet content of the lysozyme samples decreased in the presence of the ionic liquid which in turn implied that fibrillogenesis was supressed by the ionic liquid. Atomic force microscopy imaging unequivocally established that the ionic liquid attenuated fibrillogenesis in lysozyme. These results may be useful for the development of more effective therapeutics for amyloidosis.
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