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Xiao, Yi; Plakos, Kory J.I; Lou, Xinhui; White, Ryan J; Qian, Jiangrong; Plaxco, Kevin W; Soh, H. Tom
Angewandte Chemie (International ed.), June 2, 2009, Letnik: 48, Številka: 24Journal Article
Singled out for its singularity: In a single-step, single-component, fluorescence-based method for the detection of single-nucleotide polymorphisms at room temperature, the sensor is comprised of a single, self-complementary DNA strand that forms a triple-stem structure. The large conformational change that occurs upon binding to perfectly matched (PM) targets results in a significant increase in fluorescence (see picture; F=fluorophore, Q=quencher).
