DIKUL - logo
E-viri
Recenzirano Odprti dostop
  • Rapid, Sensitive, Full-Geno...
    Paden, Clinton R; Tao, Ying; Queen, Krista; Zhang, Jing; Li, Yan; Uehara, Anna; Tong, Suxiang

    Emerging infectious diseases 26, Številka: 10
    Journal Article

    We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.