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Stuiver, Maarten H.; Celis, Julio E.; van der Vliet, Peter C.
FEBS letters, April 22, 1991, Letnik: 282, Številka: 1Journal Article
Nuclear Factor IV (NFIV)is a heterodimeric DNA-binding protein from HeLa cells, recognizing molecular ends and is identical to the autoantigenic target Ku. We have identified the two NFIV/Ku subunits, by comigration, in the 2D-gel database of transformed human amnion cell (AMA) proteins. We observed that the large subunit or NFIV/Ku consists of at least 3 charge variants that correspond to SSP IEFs 5705 (81.2 kDa, p I 5.74), 6707 (81.2 kDa, p I 5.67) and 6706 (81.9 kDa, p I 5.60) in the AMA catalogue. The relative amounts of the 2 major variants (IEFs 5705 and 6707) was dependent on the state of cell proliferation. Inhibition of DNA-synthesis by hydroxyurea also changed the relative levels of the variants, whereas aphidicolin or a thymidine block had no effect. These results suggest a possible role for NFIV/Ku in DNA replication.
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in: SICRIS
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