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Serra-Batiste, Montserrat; Tolchard, James; Giusti, Fabrice; Zoonens, Manuela; Carulla, Natàlia
Frontiers in molecular biosciences, 04/2018, Letnik: 5Journal Article
We have recently reported on the preparation of a membrane-associated β Aβ42 Oligomer (β ). It corresponds to a stable and homogeneous Aβ42 oligomer that inserts into lipid bilayers as a well-defined pore and adopts a specific structure with characteristics of a β-barrel arrangement. As a follow-up of this work, we aim to establish β 's relevance in Alzheimer's disease (AD). However, β is formed under dodecyl phosphocholine (DPC) micelle conditions-intended to mimic the hydrophobic environment of membranes-which are dynamic. Consequently, dilution of the β /DPC complex in a detergent-free buffer leads to dispersion of the DPC molecules from the oligomer surface, leaving the oligomer without the hydrophobic micelle belt that stabilizes it. Since dilution is required for any biological test, transfer of β from DPC micelles into another hydrophobic biomimetic membrane environment, that remains associated with β even under high dilution conditions, is a requisite for the validation of β in AD. Here we describe conditions for exchanging DPC micelles with amphipols (APols), which are amphipathic polymers designed to stabilize membrane proteins in aqueous solutions. APols bind in an irreversible but non-covalent manner to the hydrophobic surface of membrane proteins preserving their structure even under extreme dilution conditions. We tested three types of APols with distinct physical-chemical properties and found that the β /DPC complex can only be trapped in non-ionic APols (NAPols). The characterization of the resulting β /NAPol complex by biochemical tools and structural biology techniques allowed us to establish that the oligomer structure is maintained even under high dilution. Based on these findings, this work constitutes a first step towards the validation of β in AD.
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