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Wang, Guan; Nola, Sébastien; Bovio, Simone; Bun, Philippe; Coppey-Moisan, Maïté; Lafont, Frank; Galli, Thierry
iScience, 06/2018, Letnik: 4Journal Article
The rigidity of the cell environment can vary tremendously between tissues and in pathological conditions. How this property may affect intracellular membrane dynamics is still largely unknown. Here, using atomic force microscopy, we show that cells deficient in the secretory lysosome v-SNARE VAMP7 are impaired in adaptation to substrate rigidity. Conversely, VAMP7-mediated secretion is stimulated by more rigid substrate and this regulation depends on the Longin domain of VAMP7. We further find that the Longin domain binds the kinase and retrograde trafficking adaptor LRRK1 and that LRRK1 negatively regulates VAMP7-mediated exocytosis. Conversely, VARP, a VAMP7- and kinesin 1-interacting protein, further controls the availability for secretion of peripheral VAMP7 vesicles and response of cells to mechanical constraints. LRRK1 and VARP interact with VAMP7 in a competitive manner. We propose a mechanism whereby biomechanical constraints regulate VAMP7-dependent lysosomal secretion via LRRK1 and VARP tug-of-war control of the peripheral pool of secretory lysosomes. Display omitted •VAMP7 is important for mechano-adaptation to the rigidity of cellular environment•Substrate rigidity regulates VAMP7 exocytosis•LRRK1 and VARP compete for VAMP7 binding•LRRK1 and VARP generate a tug-of-war for the transport of VAMP7 Cell Biology; Functional Aspects of Cell Biology; Biomechanics
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