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vanKuyk, Patricia A.; Benen, Jaques A. E.; Wösten, Han A. B.; Visser, Jaap; de Vries, Ronald P.
Applied microbiology and biotechnology, 01/2012, Letnik: 93, Številka: 1Journal Article
AmyR is commonly considered a regulator of starch degradation whose activity is induced by the presence of maltose, the disaccharide building block of starch. In this study, we demonstrate that the role of AmyR extends beyond starch degradation. Enzyme activity assays, genes expression analysis and growth profiling on d -glucose- and d -galactose-containing oligo- and polysaccharides showed that AmyR regulates the expression of some of the Aspergillus niger genes encoding α- and β-glucosidases, α- and β- galactosidases, as well as genes encoding α-amlyases and glucoamylases. In addition, we provide evidence that d -glucose or a metabolic product thereof may be the inducer of the AmyR system in A. niger and not maltose, as is commonly assumed.
