Phosphorus is one of the essential elements needed for the growth and reproduction of any organism. To improve the efficiency of biological phosphorus removal in sewage, it is very important to grasp the precise mechanism of biological phosphorus removal. Yeast is a single cell fungus and has a unique advantage in sewage treatment. Recent studies in the different types of yeast have revealed that there is a phosphate-responsive signal transduction (PHO) pathway to regulate phosphate-responsive genes for controlling phosphate absorption. In this review, the metabolic mechanisms and protein-protein interactions associated with the PHO pathway are highlighted firstly, and then several examples about improving the phosphorus removal efficiency of sewage by inducing gene mutation in yeast phosphorus metabolism was introduced. The aim is to provide new ideas for the realization of high-efficiency phosphorus recovery in nature.