For exploring the application of graphene nano-composite, graphene is combined with other nano-materials to develop a new graphene nano-composite with high conductivity, good biocompatibility and strong affinity for enzymes, which improved the working performance of sensors in human motion sensing. Graphene oxide (GO) is prepared by using Hummers and offeman methods, and graphene is prepared from graphene oxide with glucose as a reducing agent. On the basis of electro-deposition of Prussian blue chitosan (PB-CS) film modified gold electrode, a new nano material graphene is introduced, and glucose oxidase as a model enzyme, a glucose biosensor based on RGO/PB-CS nano-composite is constructed. The research results showed that the sensor constructed has higher sensitivity, lower detection limit and smaller apparent Michaelis constant. To sum up, the combination of graphene and Prussian blue chitosan effectively promoted the electron transfer between the electrode surface and the analytical substrate, and improved the working performance of the sensor, which has potential application value in human motion sensing.