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Park, Sang-Youl; Fung, Pauline; Nishimura, Noriyuki; Jensen, Davin R; Fujii, Hiroaki; Zhao, Yang; Lumba, Shelley; Santiago, Julia; Rodrigues, Americo; Chow, Tsz-fung F; Alfred, Simon E; Bonetta, Dario; Finkelstein, Ruth; Provart, Nicholas J; Desveaux, Darrell; Rodriguez, Pedro L; McCourt, Peter; Zhu, Jian-Kang; Schroeder, Julian I; Volkman, Brian F; Cutler, Sean R
Science (American Association for the Advancement of Science), 05/2009, Letnik: 324, Številka: 5930Journal Article
Type 2C protein phosphatases (PP2Cs) are vitally involved in abscisic acid (ABA) signaling. Here, we show that a synthetic growth inhibitor called pyrabactin functions as a selective ABA agonist. Pyrabactin acts through PYRABACTIN RESISTANCE 1 (PYR1), the founding member of a family of START proteins called PYR/PYLs, which are necessary for both pyrabactin and ABA signaling in vivo. We show that ABA binds to PYR1, which in turn binds to and inhibits PP2Cs. We conclude that PYR/PYLs are ABA receptors functioning at the apex of a negative regulatory pathway that controls ABA signaling by inhibiting PP2Cs. Our results illustrate the power of the chemical genetic approach for sidestepping genetic redundancy.
