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Perez-Sepulveda, Blanca M; Heavens, Darren; Pulford, Caisey V; Predeus, Alexander V; Low, Ross; Webster, Hermione; Dykes, Gregory F; Schudoma, Christian; Rowe, Will; Lipscombe, James; Watkins, Chris; Kumwenda, Benjamin; Shearer, Neil; Costigan, Karl; Baker, Kate S; Feasey, Nicholas A; Hinton, Jay C D; Hall, Neil
Genome Biology, 12/2021, Letnik: 22, Številka: 1Journal Article
We have developed an efficient and inexpensive pipeline for streamlining large-scale collection and genome sequencing of bacterial isolates. Evaluation of this method involved a worldwide research collaboration focused on the model organism Salmonella enterica, the 10KSG consortium. Following the optimization of a logistics pipeline that involved shipping isolates as thermolysates in ambient conditions, the project assembled a diverse collection of 10,419 isolates from low- and middle-income countries. The genomes were sequenced using the LITE pipeline for library construction, with a total reagent cost of less than USD$10 per genome. Our method can be applied to other large bacterial collections to underpin global collaborations.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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