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Ritchie, Matthew E; Dunning, Mark J; Smith, Mike L; Shi, Wei; Lynch, Andy G
PLOS computational biology/PLoS computational biology, 12/2011, Letnik: 7, Številka: 12Journal Article
Illumina whole-genome expression BeadArrays are a popular choice in gene profiling studies. Aside from the vendor-provided software tools for analyzing BeadArray expression data (GenomeStudio/BeadStudio), there exists a comprehensive set of open-source analysis tools in the Bioconductor project, many of which have been tailored to exploit the unique properties of this platform. In this article, we explore a number of these software packages and demonstrate how to perform a complete analysis of BeadArray data in various formats. The key steps of importing data, performing quality assessments, preprocessing, and annotation in the common setting of assessing differential expression in designed experiments will be covered.
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in: SICRIS
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