Abstract This paper presents the results of investigating the influence of design parameters of an electrochemical flow cell based on film interdigitated microelectrodes on its cell constant. This investigation is performed by the analytical method. To determine the cell constant the linear resistance between the adjacent fingers of the interdigitated microelectrodes is used. In its turn this resistance is found from the potential distribution in the unit subdomain of the interdigitated microelectrode system of the cell with the limited height. The dependencies of the cell constant on the cell height and the design parameters of the interdigitated microelectrode system (the spacing between fingers and the finger width) are determined. The cell height at which the change of the design parameters of the interdigitated microelectrode system has the least effect on the cell constant is found.