In this work, we present K- and L- shell fluorescence yield values of the full isonuclear sequence of Fe ions, using a state-of-the-art multiconfiguration Dirac–Fock approach. These results may be of importance for spectral fitting and plasma modeling, both in laboratory and astrophysical studies, where Fe is an important benchmark element. The K-shell fluorescence yields were found to be very similar up to the removal of 14 electrons. •We have performed K- and L- fluorescence yields of the isonuclear sequence of Fe from first principles using a multiconfiguration Dirac–Fock approach.•Changes of the fluorescence yield were assessed for K- and L- shells as a function of the charge state.•L1 subshell fluorescence yield is much more sensitive to the removal of outer electrons than the L2,3 subshell fluorescence yield.