This article aims to critically review the current state of knowledge on in vitro toxicological assessments of particulate emissions from residential biomass heating systems. The review covers various aspects of particulate matter (PM) toxicity, including oxidative stress, inflammation, genotoxicity, and cytotoxicity, all of which have important implications for understanding the development of diseases. Studies in this field have highlighted the different mechanisms that biomass combustion particles activate, which vary depending on the combustion appliances and fuels. In general, particles from conventional combustion appliances are more potent in inducing cytotoxicity, DNA damage, inflammatory responses, and oxidative stress than those from modern appliances. The sensitivity of different cell lines to the toxic effects of biomass combustion particles is also influenced by cell type and culture conditions. One of the main challenges in this field is the considerable variation in sampling strategies, sample processing, experimental conditions, assays, and extraction techniques used in biomass burning PM studies. Advanced culture systems, such as co-cultures and air-liquid interface exposures, can provide more accurate insights into the effects of biomass combustion particles compared to simpler submerged monocultures. This review provides critical insights into the complex field of toxicity from residential biomass combustion emissions, underscoring the importance of continued research and standardisation of methodologies to better understand the associated health hazards and to inform targeted interventions. Display omitted •Higher cytotoxicity, DNA damage, inflammation and ROS for traditional burning devices.•24 h exposure may not be enough to observe effects on cell endpoints.•Co-cultures and ALI exposures provide enhanced responses than submerged monocultures.•Different cell lines and experimental conditions impact endpoints discrepantly.•Transition to more standardised in vitro testing is crucial.