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Ara h 2-specific IgE epitope-like peptides inhibit the binding of IgE to Ara h 2 and suppress lgE-dependent effector cell activation [Elektronski vir]Korošec, Peter, 1970- ...Background: Clinical and experimental analyses indicate a pathognomonic role for allergen IgE crosslinking through epitope-paratope interactions as a major initial step in the cascade leading to ... effector cell activation and clinical manifestations of lgE-mediated food allergies. We aimed to undertake the initial development and assessment of Ara h 2-specific IgE epitope-like peptides that can bind to allergen-specific IgE paratopes and suppress effector cell activation. Methods: We performed biopanning, screening, IgE binding, selection and mapping of peptides. We generated synthetic peptides for use in all functional experiments. ImmunoCAP inhibition, basophil and mast cell activation tests, with LAD2 cells, a human mast cell line were performed. Twenty-six children or young adults who had peanut allergy were studied. Results: We identified and selected three linear peptides (DHPRFNRDNDVA, DHPRYGP and DHPRFST), and immunoblot analyses revealed binding to lgE from peanut-allergic individuals. The peptide sequences were aligned to the disordered region corresponding to the loop between helices 2 and 3 of Ara h 2, and conformational mapping showed that the peptides match the surface of Ara h 2 and h 6 but not other peanut allergens. In ImmunoCAP inhibition experiments, the peptides significantly inhibit the binding of IgE to Ara h 2 (p < .001). In basophil and mast cell activation tests, the peptides significantly suppressed Ara h 2-induced effector cell activation (p < .05) and increased the half-maximal Ara h 2 effective concentration (p < .05). Binding of the peptides to specific IgEs did not induce activation of basophils or mast cells. Conclusions: These studies show that the indicated peptides reduce the allergenic activity of Ara h 2 and suppress lgE-dependent basophil and mast cell activation. These observations may suggest a novel therapeutic strategy for food allergy based on epitope-paratop blocking.Source: Clinical & experimental allergy [Elektronski vir]. - ISSN 1365-2222 (Vol. 53, iss. 6, Jun. 2023, str. 636-647)Type of material - e-article ; adult, seriousPublish date - 2023Language - englishCOBISS.SI-ID - 151992835
Author
Korošec, Peter, 1970- |
Koren, Ana, biotehnologinja |
Debeljak, Jerneja |
Zahirović, Abida |
Skerbinjek-Kavalar, Maja |
Berce, Vojko |
Dejanović, Luka |
Luzar, Jernej, farmacevt |
Štrukelj, Borut |
Lunder, Mojca, 1977-
Topics
Alergeni |
Bazofilci |
Imunoglobulini |
Alergija |
Ara h 2 |
test aktivacije bazofilcev |
epitopi |
test aktivacije mastocitov |
alergija za arašide |
peptidi |
specifični IgE |
Ara h 2 |
immunoCAP inhibition |
basophil activation test |
epitopes |
mast cell activation test |
paratopes |
peanut allergy |
peptides |
specific IgE
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Author | Korošec, Peter, 1970- ... |
Title | Ara h 2-specific IgE epitope-like peptides inhibit the binding of IgE to Ara h 2 and suppress lgE-dependent effector cell activation [Elektronski vir] |
Publication date | |
COBISS.SI-ID | 151992835 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Celostna obravnava alergijskih bolezni in astme v Sloveniji: od epidemiologije do genetike | P3-0360-2019 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Farmacevtska biotehnologija: znanost za zdravje | P4-0127-2019 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Korošec, Peter, 1970- | 22807 |
Koren, Ana, biotehnologinja | 34101 |
Debeljak, Jerneja | 51978 |
Zahirović, Abida | 52419 |
Skerbinjek-Kavalar, Maja | 21876 |
Berce, Vojko | 33666 |
Dejanović, Luka | 56324 |
Luzar, Jernej, farmacevt | 35434 |
Štrukelj, Borut | 07849 |
Lunder, Mojca, 1977- | 25096 |
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