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Real-time PCR genotyping of human platelet alloantigens HPA-1, HPA-2, HPA-3 and HPA-5 is superior to the standard PCR-SSP methodFicko Trček, Tanja ...Genotyping of the human platelet alloantigens (HPA) is useful for the diagnosis and therapy of the patients with alloimmune thrombocytopenic syndromes, such as post-transfusion refractoriness to ... platelets, post-transfusion thrombocytopenic purpura and foetomaternal alloimmune thrombocytopenia. We have developed, optimized and validated a new method for simultaneous genotyping of HPAs - HPA-1, HPA-2, HPA-3 and HPA-5 - by using thereal-time polymerase chain reaction (PCR) based on TaqMan technology. Its performances were compared to those of the standard PCR-sequence-specific primers (SSP) method by testing 120 DNA samples. Several discrepancies betweenthe two methods have been observed, especially in the HPA-3 genotyping.Evidently, the PCRSSP method produced several false positive results due to its technical drawbacks. Based on our comparison, we believe that the new real-time TaqMan PCR assay for the HPA-1, HPA-2, HPA-3 and HPA-5 genotyping is faster, more reliable and reproducible, compared to the standardPCR-SSP.Source: Transfusion medicine. - ISSN 0958-7578 (Letn. 14, 2004, str. 425-432)Type of material - article, component partPublish date - 2004Language - englishCOBISS.SI-ID - 18785497
Author
Ficko Trček, Tanja |
Galvani, Vesna |
Rupreht, Ruth |
Dovč, Tadeja |
Rožman, Primož, 1954-
Topics
Antigens, Human Platelet |
Thrombocytopenia |
Diagnosis |
Genotype |
Blood Donors |
Polymerase Chain Reaction |
Sequence Analysis, Dna |
Antigeni človeških trombocitov |
Genotip |
Trombocitopenija |
Krvodajalci |
Polimerazna, verižna reakcija |
Zaporedje, analiza, DNA
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Ficko Trček, Tanja | 20606 |
Galvani, Vesna | 15455 |
Rupreht, Ruth | 15103 |
Dovč, Tadeja | 20605 |
Rožman, Primož, 1954- | 12336 |
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