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Kontrola kvalitete DNA mikromrež nizke gostote = Quality control of low-density DNA microarrays
Devjak, Rok ...
DNA microarray technology, showing great perspective in clinical diagnostics and prognostics, is facing a problem of loosely defined and non-unified data quality control. We have developed a general ...approach for controlling the quality of low density DNA microarrays, which is based on the analysis of covariance of control probes. We have applied the approach to the Steroltalk microarray, which was developed to study genes in connection with cholesterol homeostasis. We analyzed changes in genes expression in human primary hepatocytes treated with rifampicin and rosuvastatin and evaluated the approach by comparing our results to these from the literature. The proposed approach is independent of the type of DNA microarray and therefore may contribute to more rapid transfer of this technology to the clinical practice.
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