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Antigenska in molekularna določitev humanih rotavirusov : diplomska naloga = Antigenic and molecular determination of human rotaviruses : graduation thesis
Koblič, Andreja
Rotavirusi so pomembnejǴi virusni enteropatogeni in predstavljajo glavni vzrokza akutno drisko pri otrocih. Vzorce iztrebkov smo dobili od otrok z akutnim gastroenteritisom, pri katerih smo z ...encimsko imunskim testom ali elektronsko mikroskopijo ugotovili rotavirusno okuÍbo. Z encimsko imunskim testom smo dolořili skupinsko specifiřen antigen (VP6) za rotaviruse skupine A. Z veriÍno reakcijo s polimerazo smo iz vzorcev iztrebka pomnoÍili odsek gena, ki kodira osnovni zunanji kapsidni glikoprotein VP7. Iz vzorcev iztrebkov smo izolirali dvovijařno RNA in jo uporabili kot matrico v reverzni transkripciji s pomnoÍevanjem, kjer smo uporabili zařetni oligonukleotid, s katerim smo pomnoÍili celoten odsek. Z uporabo Ǵestih tipsko specifiřnih zařetnih oligonukleotidov, ki pomnoÍijo le dolořeno podrořje celotnega gena 9,smo dolořili serotipe G. V obdobju jesen/zima 1999/2000 smo pri vseh vzorcih(46) dolořili serotip G1, pri vzorcih iz leta 1993 serotipa G1 in G4 (9 vzorcev spada v serotip G1, 5 vzorcev spada v serotip G4 in 6 vzorcev predstavlja serotip G1 in G4).
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [A. Koblič] ; [BF, Enota medoddelčnega študija mikrobiologije], 2000
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