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Expression analysis of all protease genes reveals cathepsin K to be overexpressed in glioblastoma [Elektronski vir]Verbovšek, Urška, 1985- ...Background: Cancer genome and transcriptome analyses advanced our understanding of cancer biology. We performed transcriptome analysis of all known genes of peptidases also called proteases and their ... endogenous inhibitors in glioblastoma multiforme (GBM), which is one of the most aggressive and deadly types of brain cancers, where unbalanced proteolysis is associated with tumor progression. Methods: Comparisons were performed between the transcriptomics of primary GBM tumors and unmatched nonmalignant brain tissue, and between GBM cell lines (U87-MG and U373) and a control human astrocyte cell line (NHA). Publicly-available data sets and our own datasets were integrated and normalized using bioinformatics tools to reveal protease and protease inhibitor genes with deregulated expression in both malignant versus non-malignant tissues and cells. Results: Of the 311 protease genes identified to be differentially expressed in both GBM tissues and cells, 5 genes were highly overexpressed, 2 genes coding for non-peptidase homologues transferrin receptor (TFRC) and G protein-coupled receptor 56 (GPR56), as well as 3 genes coding for the proteases endoplasmic reticulum aminopeptidase 2 (ERAP2), glutamine-fructose-6-phosphate transaminase 2 (GFPT2) and cathepsin K (CTSK), whereas one gene, that of the serine protease carboxypeptidase E (CPE) was strongly reduced in expression. Seventy five protease inhibitor genes were differentially expressed, of which 3 genes were highly overexpressed, the genes coding for stefin B (CSTB), peptidase inhibitor 3 (PI3 also named elafin) and CD74. Seven out of 8 genes (except CSTB) were validated using RT-qPCR in GBM cell lines. CTSK overexpression was validated using RT-qPCR in GBM tissues as well. Cathepsin K immunohistochemical staining and western blotting showed that only proteolytically inactive proforms of cathepsin K were overexpressed in GBM tissues and cells. Conclusions: The presence of high levels of inactive proforms of cathepsin K in GBM tissues and cells indicate that in GBM the proteolytic/collagenolytic role is not its primary function but it plays rather a different yet unknown role.Source: PloS one [Elektronski vir]. - ISSN 1932-6203 (Vol. 9, iss. 10, 2014, str. 1- 12)Type of material - e-article ; adult, seriousPublish date - 2014Language - englishCOBISS.SI-ID - 3237711
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Author | Verbovšek, Urška, 1985- ... |
Title | Expression analysis of all protease genes reveals cathepsin K to be overexpressed in glioblastoma [Elektronski vir] |
Publication date | 2014-10-30 |
COBISS.SI-ID | 3237711 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
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Ekotoksiologija, toksikološka genomika in karcinogeneza | P1-0245-2009 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Verbovšek, Urška, 1985- | 32811 |
Motaln, Helena | 21397 |
Rotter, Ana | 27503 |
Atai, Nadia A. | ![]() |
Gruden, Kristina | 12688 |
Noorden, Cornelis J. F. van | 51349 |
Lah Turnšek, Tamara | 07802 |
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