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Parameter optimization for the enzymatic hydrolysis of sunflower oil in high-pressure reactors
Primožič, Mateja, 1975- ; Leitgeb, Maja ; Knez, Željko
This study is concerned with the hydrolysis of sunflower oil in the presence of lipase preparation Lipolase 100T (Aspergillus niger lipase). Supercritical carbon dioxide was used as a solvent for ...this reaction. In a high-pressure stirred tank reactor operated in a batch mode, the effects of various process parameters (temperature, pressure, enzyme-substrate ratio, pH, and oil-buffer ratio) were investigated to determine the optimal reaction rate and conversionfor the hydrolysis process. The optimal concentration of lipase was 0.0714 g/mL of CO2-free reaction mixture, and the highest conversions of oleic acid (0.193 g/g of oil phase) and linoleic acid (0.586 g/g of oil phase) were obtained at 50°C, 200 bar, pH = 7, and an oil/buffer ratio of 1/1 (w/w).
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