BF, Central Biotechnical Library, Lj. (BFCBK)
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Uporaba genskih označevalcev v žlahtnjenju jablan (Malus domestica Borkh.) : doktorska disertacija = The use of genetic markers for apple (Malus domestica Borkh.) breeding : dissertation thesisViršček-Marn, MojcaV nalogi je bila proučevana uporabnost RAPD tehnike za povečanje učinkovitosti žlahtnjenja jablan. Pregled namnoževanja v literaturi objavljenih RAPD markerjev OPM18/900, OPD20/600 in OPA 15/900, ... vezanih za Vf gen za odpornost proti škrlupu, pri 28 proti škrlupu odpornih žlahtnih sort jablan, 4 originalnih virih genov za odpornost iz rodu Malus in 10 občutljivih žlahtnih kultivarjih po različnih protokolih je pokazal dobro ponovljivost preizkušanih markerjev med laboratoriji ne glede na uporabljen protokol, primerjave celotnih RAPD vzorcev istih kultivarjev pa potrjujejo občutljivost RAPD tehnike na spremembe v koncentraciji in vrsti sestavin reakcijske mešanice in temperaturnih vzorcev. Rezultati analiz tudi kažejo, da se geni Va, Vr in Vm za odpornost proti škrlupu dedujejo neodvisno od Vf gena in markerskih fragmentov OPM 18/900, OPD20/600 in OPA15/900, zato so vsi trije preizkušani markerji uporabni za zgodnjo selekcijo na Vf gen tudi pri vzgoji odpornih sort s kopičenjem več genov za odpornost. Proučevanje regenerantov iz listov in vitro gojenih jablanovih sort 'zlati delišes bovey' in 'goldspur' je pokazalo velik obseg somaklonalne variabilnosti , saj smo pri pregledu samo 0.01% genoma z RAPD markerji med skupno 77 regeneranti odkrili 3 s spremenjenimi RAPD vzorci. Rezultati kažejo, da je adventivna organogeneza zanimiva tehnika za vzgojo mutantov jablanovih sort z izboljšanimi lastnostmi.Type of material - dissertation ; adult, seriousPublication and manufacture - Ljubljana : [M. Viršček-Marn], 1996Language - slovenianCOBISS.SI-ID - 51577
Author
Viršček-Marn, Mojca
Other authors
Javornik, Branka
Topics
sadjarstvo |
jablane |
Malus domestica Borkh. |
žlahtnenje |
RAPD tehnika |
molekularni markerji |
ponovljivost |
škrlup |
Venturia inaequalis |
odpornost |
somaklonalna variabilnost |
adventivna organogeneza
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BF, Central Biotechnical Library, Lj. |
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Skladišče DR II 0000009042 IN: 01996055619 Skladišče DR II 9042 IN: 01996055619 |
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Database name | Field | Year |
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Viršček-Marn, Mojca | 03853 |
Javornik, Branka | 05994 |
Source: Personal bibliographies
and: SICRIS
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