BF, Central Biotechnical Library, Lj. (BFCBK)
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Vegetativno razmnoževanje "in vitro" Malus domestica Borkh. cv. majda v primerjavi s cv. zlati delišes = Vegetative propagation "in vitro" of the Malus domestica Borkh. cv. majda in comparison with the cv. golden delicious : magistrska nalogaViršček-Marn, MojcaIzvedli smo 4 poskuse v stopnji razmnoževanja in 3 v stopnji ukoreninjenja poganjkov. V poskusih razmnoževanja razlike med kultivarjema niso bile statistično značilne ali pa je cv. majda boljše rasla ... in se razmnoževala od cv. zlati delišes. Dobro rast in razmnoževanje smo pri obeh kultivarjih dosegli pri 50-60-dnevnem gojenju na trdnem hranilnem mediju z makro-in mikroelementi po MS, 100 mg/l mio-inositola, 2 mg/l tiamin-HCl, 1 mg/l piridoksin-HCl, 1 mg/l nikotinske kisline, 30 g/l saharoze, 6 g/l agarja (BDH) in 1 mg/l BA, ki smo mu po cca. 30 dneh dodali 10 ml tekočega medija iste sestave. Pri cv. majda sta bili rast in razmnoževanje boljši na mediju z normalno koncentracijo makro- in mikroelementov po MS, pri cv. zlati delišes pa na mediju s polovično koncentracijo. Dodajanje IBA (0,1 mg/l ali 1 mg/l) in KIN (1 mg/l) v hranilni medij z 1 mg/l BA ni statistično značilno vplivalo na rast in razmnoževanje obeh kultivarjev. Razmnoževanje obeh kultivarjev na mediju z 1 mg/l KIN je bilo izredno slabo, maloštevilni poganjki pa so se lepo razvili. Cv. majda je bila v vseh poskusih ukoreninjenja po odstotku ukoreninjenja statistično značilno boljša od cv. zlati delišes. Najboljše rezultate smo dosegli pri 6-dnevni indukciji zasnove korenin na trdnem hranilnem mediju z 1 mg/l IBA, ki ji je sledilo 15-dnevno gojenje na mediju brez hormonov. Pri 21-dnevni indukciji zasnove korenin z 1 mg/l IBA so se razvile zelo kratke korenine, obdane s kalusom. Sestava medija za ukoreninjenje je bila z izjemo hormonov enaka kot pri mediju za razmnoževanje poganjkov.Type of material - master's thesis ; adult, seriousPublication and manufacture - Ljubljana : [M. Marn], 1988Language - slovenianCOBISS.SI-ID - 87929
Author
Viršček-Marn, Mojca
Other authors
Kreft, Ivan, 1941-
Topics
sadjarstvo |
jablane |
Malus domestica Borkh. |
kultivarji |
vegetativno razmnoževanje |
tkivne kulture |
tkivne kulture
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Database name | Field | Year |
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Viršček-Marn, Mojca | 03853 |
Kreft, Ivan, 1941- | 00950 |
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