-
In vitro mutagenesis of the GGS1/TPS1 gene of Sacharomyces cerevisiae : graduation thesis = In vitro mutacija gena GGS1/TPS1 kvasovke Saccharomyces cerevisiae : diplomska nalogaBuzeti, TatjanaIn vitro mutacije na genu GGS1/TPS1 kvasovke Saccharomyces cerevisiae so potekale z naključno in s ciljano mutagenezo. Rezultat ciljane mutageneze so bile 3 mutacije, D395A, H185A in N295A. S ... plazmidi Ycplac33/TPS1, ki so vsebovali mutagene alele, sem transformirala seve W303-1Atps1[delta] in W303-1Atps1[delta]tps2[delta]. Na podlagi opazovanja fenotipskih sprememb mutantov na selektivnem gojišču sem opazovala spremembo v glikolitičnem fluksu oz. njihovo rast na glukozi ter termotoleranco. Aktivnost trehaloza-6-fosfat sintaze (aktivnost Tps1) in vsebnost trehaloze sem spremljala z biokemičnimi metodami. Pri vseh treh mutacijah je bila popolnoma uničena aktivnost Tps1, posledica česar je tudi minimalna vsebnost trehaloze v primeru mutacij D395A in H185A. V primeru mutacije N295A je bila vsebnost trehaloze nekoliko višja, s čimer bi lahko razlagali še vedno obstoječo regulacijo glukoznega fluksa v glikolizo in s tem rast na glukoznem gojišču. Rezultati potrjujejo vlogo gena GGS1/TPS1 v glikolitičnem fluksu, vendar ne moremo potrditi niti zavreči nobene izmed treh predlaganih razlag njegove regulatorne vloge v omenjeni metabolni poti.Type of material - undergraduate thesis ; adult, seriousPublication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [T. Buzeti], 1999Language - englishCOBISS.SI-ID - 2262904
Author
Buzeti, Tatjana
Other authors
Raspor, Peter |
Smole Možina, Sonja
Topics
Saccharomyces cerevisiae |
mutacije |
Gen GGS1/TPS1 |
trehaloza-6-fosfat sintaza |
trehaloza |
glikoliza |
termotoleranca mutantov |
termotoleranca mutantov |
Saccharomyces cerevisiae |
mutations |
GGS1/TPS1 gene |
trehalose-6-phosphate synthase |
trehalose |
glycolysis |
thermotolerance of the mutants |
thermotolerance of the mutants
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Reserve material at the desired pickup location.
Pickup location |
Material status | Reservation |
---|---|---|
BF, Dept. of Food Science and Technology, Lj. |
available - outside loan, loan period: 1 months
|
Call number – location, accession no. ... |
Copy status |
---|---|
Hodnik DN 0000000915 IN: 0011570 Hodnik DN 915 IN: 0011570 |
available - outside loan, loan period: 1 months
|
Hodnik DN 0000000915 a IN: 0011571 Hodnik DN 915 a IN: 0011571 |
available - outside loan, loan period: 1 months
|
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Raspor, Peter | 04001 |
Smole Možina, Sonja | 07030 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.
Izposojeno gradivo lahko vrnete tudi v nabiralnik pred knjižnico.