BF, Dept. of Animal Science, Ljubljana (BFZOO)
-
Mycoplasma gallisepticum and Mycoplasma synoviae express a cysteine protease CysP, which can cleave chicken IgG into Fab and FcCizelj, Ivanka ...Major poultry pathogens M. gallisepticum and M. synoviae share a gene encoding a putative cysteine protease CysP similar to papain cysteine (CaA subfamily). Comparison of the cysP gene sequences of ... 18 M. synoviae and 10 M. gallisepticum strains sequenced in this study showed polymorphisms, including deletions. Seven M. synoviae strains, including the type strain WVU 1853, had a 39 bp deletion in the 3' end of the cysP gene. In the same cysP region, all M. gallisepticum strains showed a deletion of 66bp. Immunoblot analysis with specific antibpodies demonstrated that M. synoviae strains expressed CysP, which was approximately 65 kDa. both M. synoviae and M. gallisepticum were able to digest chicken IgG (cIgG). Incubation of cIgG (-170 kDa) with M. synoviae or M. gallisepticum cells (-15 h at 37oC) resultes in a papain-like cleavage pattern of cIgG and fragments corresponding to the antigenbinding fragment of IgG (Fab, -45kDa) and the crystallizable region fragment (Fc) of the IgG heavy chain (dimer of -60 kDa). Iodoacetamide (50 mM) prevented cleavage of cIgG by both Mycoplasma species. Following site-directed mutagenesis (eight TGA codons were changed to TGG) the cysP gene of M. synoviae ULB 925 was expressed as a His-taggede protein in a cell-free system. Purified recombinant CysP (rCysp; -67kDa, pI-8) cleaved cIgG into Fab and Fc fragments. This indicates that CysP is responsible for the cIgG cleavage caused by M. synoviae and probbaly, by M. gallisepticum. This is the first evidence to our knowledge that mycopasmas have enzymes that can cleave the host IgG and indicates a novel strategy used by M. gallisepticum and M. synoviae for prolomged survival despite the antibody response of their host.Source: Microbiology. - ISSN 1350-0872 (Vol. 157, no. 2, 2011, str. 362-372)Type of material - article, component partPublish date - 2011Language - englishCOBISS.SI-ID - 2835848
Author
Cizelj, Ivanka |
Berčič, Rebeka Lucijana |
Dušanić, Daliborka |
Narat, Mojca |
Kos, Janko, 1959- |
Dovč, Peter |
Benčina, Dušan
Topics
mikrobiologija |
mikoplazme |
Mycoplasma synoviae |
Mycoplasma gallisepticum |
encimi |
cistin |
proteaze
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Cizelj, Ivanka | 29430 |
Berčič, Rebeka Lucijana | 27545 |
Dušanić, Daliborka | 29431 |
Narat, Mojca | 05008 |
Kos, Janko, 1959- | 04648 |
Dovč, Peter | 05098 |
Benčina, Dušan | 07914 |
Source: Personal bibliographies
and: SICRIS
Select pickup location:
Material pickup by post
Delivery address:
Address is missing from the member's data.
The address retrieval service is currently unavailable, please try again.
By clicking the "OK" button, you will confirm the pickup location selected above and complete the reservation process.
By clicking the "OK" button, you will confirm the above pickup location and delivery address, and complete the reservation process.
By clicking the "OK" button, you will confirm the address selected above and complete the reservation process.
Notification
Automatic login and reservation service currently not available. You can reserve the material on the Biblos portal or try again here later.
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
Pickup location | Material status | Reservation |
---|
Reservation in progress
Please wait a moment.
Reservation was successful.
Reservation failed.
Reservation...
Membership card:
Pickup location: