MF, Central Medical Library, Lj. (CMK)
Urnik v septembru: ponedeljek, torek in četrtek: 8.00 do 19.00, sreda in petek: 8.00 do 16.00. Izjemoma bo 5. 9. in 16. 9., knjižnica odprta med 8.00 in 16.00.
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Platelet activation and blood extracellular vesicles : the influence of venepuncture and short blood storageMarić, Ivica ...Extracellular vesicles (EVs) as membrane-bound particles released by various cells are potential tools for diagnosis and treatment. Blood cells, particularly platelets, are the source of circulating ... EVs. Material: EVs were enriched with gradient ultracentrifugation and measured by nanoparticle tracking assay. A flow cytometric multiplex assay was used for cellular source determination. Activation of platelets was measured as a percentage of CD62p+/CD61+ platelets and correlated with the concentration and size of released EVs. Results: In general there was no statistically significant correlation between EVs` concentration and degree of platelet activation. EVs from different cellular sources were detected. Comparing different needle thicknesses, there was a decrease in the EVs concentration for the 16G needle versus the 21G needle, but no difference was observed for EVs` size and phenotype or platelets activation. During blood storage, platelet activation increased, but there was no effect on the EVs` concentration, size, or phenotype. Conclusions: Preanalytical factors like needle thickness and storage time can affect the MVs' properties. Activation of platelets during blood collection or blood storage occurs; however, it is difficult to determine its effect on the physiological properties of EVs since the mechanisms of EVs` biogenesis and especially clearness are not precisely known.Source: Blood cells, molecules & diseases. - ISSN 1079-9796 (Vol. 106, iss. [article no.] 102842, 2024, str. 1-7)Type of material - article, component part ; adult, seriousPublish date - 2024Language - englishCOBISS.SI-ID - 192381699
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source: Blood cells, molecules & diseases. - ISSN 1079-9796 (Vol. 106, iss. [article no.] 102842, 2024, str. 1-7)
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Marić, Ivica | ![]() |
Žiberna, Klemen | 56196 |
Kolenc, Ana, biotehnologija, 1998- | ![]() |
Maličev, Elvira | 21228 |
Source: Personal bibliographies
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