Faculty of Pharmacy, Lj. (FFALJ)
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Ugotavljanje citotoksičnosti acikličnih desmuramil dipeptidov = Determination of cytotoxic activity of acyclic desmuramyl dipeptides : diplomska nalogaKuhar, Polonca, 1977V moji diplomski nalogi smo ugotavljali citotoksièno delovanje acikliènih desmuramil dipeptidov, ki so bili sintetizirani na Fakulteti za farmacijo za ugotavljanje povezave med strukturo in ... imunomodulatornim delovanjem. Spojine so sestavljene iz dveh strukturnih enotČ iz derivatov muramil dipeptida in adamantilnega ali ftalimidnega dela. Za testiranje citotoksiènosti smo se odloèili, ker smo v literaturi zasledili podatke o protitumornem delovanju spojin, ki so vsebovale podobne strukturne elemente. Testirane vzorce so predstavljale sterilne vodne raztopine izbranih spojin v koncentraciji 10-4 M v 0,1% DMSO. Uporabili smo tri tumorske celiène linijeČ celice tumorja dojke, jetrnega hepatoma in karcinoma pljuè ter celièno linijo normalnih epitelijskih celic. Za ugotavljanje vpliva spojin na rast in razmnozevanje posameznih celiènih linij smo uporabili MTT test, kolorimetriènometodo, s katero smo merili metabolièno aktivnost celic. Izvedlismo ga tako, da smo celice izpostavili vplivu spojin za 24 ur v kontroliranih pogojihČ 5 % CO2, 37°C. Kontrolni vzorec je vseboval celice, izpostavljene samo mediju z 0,1% DMSO. Celice, ki so prezivele in ohranile metabolièno aktivnost, so bile sposobne pretvorbe reagenta MTT v vijolièno obarvane formazanske kristale. Le-te smo raztopili v DMSO in pomerili absorbanco, ki je sorazmerna stevilu zivih celic, pri valovnih dolzinah 570in 690 nm. Absorbanco kontrolnih celic smo primerjali z absorbanco celic, ki so bile izpostavljene testnim spojinam. Rezultate smo statistièno obdelali in s Studentovim testom za vsako spojino izraèunali, èe je njen vpliv na posamezno tumorsko linijo statistièno znaèilen. Nekatere spojine so pokazale citotoksièno delovanje na tumorske linije, ki pa je bilo sibko (najbolj, za 23%, je prezivelost zmanjsala spojina USG 1) v primerjavi s kontrolnim vzorcem. Na normalne celice ni imela statistièno znaèilnega vpliva nobena spojina. Tri spojine so povzroèile znaèilno inhibicijo pri vseh treh tumorskihlinijah.Èeprav je bila ugotovljena doloèena stopnja inhibicije rasti in razmnozevanja celic tumorskih linij, je bil ta uèinek presibak, da bi bilo smiselno substance naprej razvijati v smeri protitumornih uèinkovin. Ker pa spojine niso znaèilno vplivale na rast normalnih epitelijskih celic, jih lahkonaprej razvijamo v smeri potencialnih imunomodulatorjev.Type of material - undergr. thesisPublication and manufacture - Ljubljana : [Kuhar P.], 2002Language - slovenianCOBISS.SI-ID - 1131121
Author
Kuhar, Polonca, 1977
Other authors
Gobec, Stanislav |
Filipič, Metka, 1954-
Topics
desmuramildipeptidi |
citotoksično delovanje |
MTT test |
gojenje celic |
ftalimidi |
adamantani
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Knjižnica dp 0000000547 Kuhar Polonca Ugotavljanje IN: 0006280 Knjižnica dp 547 Kuhar Polonca Ugotavljanje IN: 0006280 |
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Filipič, Metka, 1954- | 09892 |
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