Faculty of Pharmacy, Lj. (FFALJ)
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Uvedba mDx hemavision reagenčnega kompleta za molekularno genetično prepoznavanje levkemij = Detection of specific chromosal rearrangement in leukemia patients by mdx hemavision kit : diplomska nalogaLjutić, ElviraV zadnjih letih se je izkazalo, da raziskava kromosomov doprinese k natanèni diagnozi in oceni prognoze levkemij. Pri veèini levkemij lahko odkrijemo v klonu malignih celic kromosomske preureditve. ... Pomen teh se kaze v razvrstitvi Svetovne zdravstvene organizacije za maligne novotvorbe krvotvornega sistema, ki vkljuèuje in povezuje morfoloske, citogenetiène, molekularno genetiène, imunoloske in kliniène lastnosti bolezni. Obratno prepisovanje in hkratna verizna reakcija s polimerazo (angl. reverse transcriptase-polymerase chain reactionč RT-PCR) ima vse veèji pomen pri diagnostiki akutnih levkemij in kronièno mieloiène levkemije, saj omogoèa ugotavljanje specifiènih translokacij, ki so znaèilne za posamezne podvrste levkemij. S to reakcijo lahko doloèimo prisotnost ali odsotnost specifiènih mRNK prepisov oziroma cDNKsegmentov po obratnem prepisovanju zdruzenih ali okvarjenih genov, ki nastanejo zaradi kromosomskih preureditev. Po usklajenem RT-PCR protokolu smo ugotovili mRNK, prepise znaèilne za translokacije t(9č22)(q34čq11), t(8č21)(q22čq22), inv 16(p13čq22), t(15č17)(q21čq21) in t(4č11)(q21čq23). Za kvalitativno ugotavljanje 28 razliènih translokacij ali kromosomskih preureditev, znaèilnih za posamezne podvrste levkemij, smo uporabili mDx HemaVision reagenèni komplet, z naèinom obratnega prepisovanja in hkratne in vgnezdene verizne reakcije s polimerazo. Uporabnost mDx HemaVision reagenènegakompleta smo se odloèili preveriti v 26 vzorcih RNK bolnikov z akutno levkemijo in 7 s kronièno mieloproliferativno boleznijo, ki smo jih izbrali v èasovnem obdobju od 09. 01.-07. 05. 2003. Dobili smo sto odstotno ujemanje rezultatov z rezultati, dobljenimi po usklajenem RT-PCR protokolu. Z uporabo mDx HemaVision reagenènega kompleta smo pri vzorcu bolnika (G.S.) dobili mRNK prepis t(10č11)(p21čq23), ki je znaèilen za akutno mieloièno levkemijo. Na osnovi skladnosti dobljenih rezultatov sklepamo, da je metoda primerna za molekularno genetièno prepoznavanje levkemij, saj omogoèa ugotovitev velikega stevila specifiènih mRNK prepisov pri kromosmomskih nepravilnostih soèasno. S tem pridobimo veè informacij v krajsem èasu in se lahko uporablja kot pomembna osnovna preiskava.Type of material - undergraduate thesisPublication and manufacture - Ljubljana : [Ljutić, E.], 2004Language - slovenianCOBISS.SI-ID - 1536113
Author
Ljutić, Elvira
Other authors
Černelč, Peter |
Pajič, Tadej, 1969-
Topics
RNK |
mDx hemavision |
RT-PCR protokoli |
gelska elektroforeza |
maligne novotvorbe |
diagnostika
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Knjižnica dp 0000000061 Ljutić Elvira Uvedba IN: 0007671 Knjižnica dp 61 Ljutić Elvira Uvedba IN: 0007671 |
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Database name | Field | Year |
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Ljutić, Elvira | ![]() |
Černelč, Peter | 07012 |
Pajič, Tadej, 1969- | 23818 |
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