Faculty of Pharmacy, Lj. (FFALJ)
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Ocena limfocitičnih podvrst s pomočjo električnih in optičnih načinov detekcij pri bolnikih z malignimi limfomi = Evaluation of lymphocyte subpopulatin with electrical and optical detection method at the patients with malignant lymphoma : diplomska nalogaBohinc, NatašaCoulter LH 750 (Beckman Coulter) in ADVIA 120 (Bayer Diagnostics) sta avtomatizirana hematoloska analizatorja, ki delujeta na principu pretoène citometrije ter imata razliène izvedbe detekcije ... osnovnih hematoloskih parametrov. Coulter LH 750 deluje na principu elektro-optiène detekcije s pomoèjo VCS tehnologije, ADVIA 120 pa na principu optiène detekcije z barvanjem s peroksidazo.Vrednotenje analizatorjev smo preiskovali z doloèanjemlimfocitov pri bolnikih z limfoprolierativnimi boleznimi zrelih celic B. V nasem primeru je bilo to 35 vzorcev bolnikov z kronièno limfocitno levkemijo, 9 z limfomom obrobnih celic vranice, 2 vzorca bolnikov z limfoplazmocitnim imunocitomom, 2 z limfomom plasènih celic in vzorec bolnika z dlakastocelièno levkemijo. Za te bolezni so znaèilne razliène razvojne stopnje limfocitov in s tem razlièna zgradba.Nalogo smo razdelili na dva cilja. V prvem smo vrednotili VCS tehnologijo. Merili smo srednje vrednosti volumna, prevodnosti in razprsenosti svetlobe limfocitov pri skupini bolnikov,ki smo jo primerjali s skupino zdravih preiskovancev. Ugotovili smo razliène velikosti limfocitov pri bolnikih v primerjavi z zdravimi preiskovanci. Ker smo imeli velik odklon od srednje vrednosti pri skupini bolnikov, smo prisli do zakljuèka, da imamo v vzorcih bolnikov prisotnih veè populacij limfocitov.Drugi cilj pa je bil ugotoviti morebitne razlike med rezultati dveh analizatorjev. Primerjali smo stevilo levkocitov absolutno stevilèno koncentracijo limfocitov in monocitov ter delez limfocitov in monocitov. Razlike med analizatorjema so se pokazale predvsem zaradi tega, kerje analizator Coulter LH 750 atipiène velike limfocite vmestil v monocite. S tem smo dobili veèje stevilo monocitov, kar smo primerjali z oceno odstotka levkocitov v krvnem razmazu. Analizator ADVIA 120 pa je skupino celic uvrstil na posebno mesto v diagramu, ki ima oznako velike neobarvane celice. Sem je uvrstil velike limfocite, ker se v reakciji s peroksidazo ne barvajo. Tako imamo dobro ujemanje odstotka monocitov med ADVIA 120 in diferencialno krvno sliko ocenjeno z mikroskopom. Res je, da je pridobivanje rezultatov z analizatorji hitrejse, poraba reagentov in bioloskega materijala sta manjsi, odmerjanje reagentov je natanènejse, vendar èe imamo v vzorcih bodisi limfomske celice bodisi, da je prisoten sum na drugo krvno bolezen, moramo vzorec pregledati se pod mikroskopom. Diagnozo pa lahko postavimo sele, ko imamo pozitivne rezultate tudi drugih usmerjenih preiskav za doloèeno bolezen.Type of material - undergraduate thesisPublication and manufacture - Ljubljana : [Bohinc, N.], 2006Language - slovenianCOBISS.SI-ID - 1929329
Author
Bohinc, Nataša
Other authors
Černelč, Peter |
Pajič, Tadej, 1969-
Topics
limfociti |
morfologija |
bolezni zrelih celic B |
reagenti |
pretočna citometrija |
hematološki analizatorji
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Knjižnica dp 0000000061 Bohinc Nataša Ocena IN: 0008938 Knjižnica dp 61 Bohinc Nataša Ocena IN: 0008938 |
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Bohinc, Nataša | ![]() |
Černelč, Peter | 07012 |
Pajič, Tadej, 1969- | 23818 |
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