Faculty of Pharmacy, Lj. (FFALJ)
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Vrednotenje celične linije PC-12 s pretočno citometrijo s sočasnim zajemanjem slike = Evaluating of PC-12 cell line using imaging flow cytometry : univerzitetni študijski program KozmetologijaLipovnik, AnjaV diplomskem delu smo vrednotili celično linijo PC-12, ki predstavlja dober nevrološki model za raziskavo nevrodegenerativnih bolezni. Analize smo opravili s pretočno citometrijo s sočasnim ... zajemanjem slike. Slikovni citometer ImageStream združuje kovencionalno pretočno citometrijo in sodobno fluorescenčno mikroskopijo. Omogoča multiparametrične analize, s katerimi lahko hitro in učinkovito karakteriziramo lastnosti posameznih celic, kot so velikost, oblika, tekstura, granuliranost in intenzivnost fluorescence. Celice smo označili s fluorofornimi označevalci (DAPI, Alexa Fluor 488, Alexa Fluor 633, TRITC) neposredno, s faloidinom ali posredno s protitelesi. S pomočjo programske opreme IDEAS preučili delež živih celic PC-12 ter distribucijo aktina, cistatina C in katepsina X. S statistično analizo (določitev vrednost RD) smo na podlagi fenotipskih značilnosti celic ovrednotili subpopulacije in posamezne celice. Intenziteto fluorescence smo določili pri permeabiliziranih in nepermeabiliziranih celicah. Višje vrednosti intenzitete signala smo zaznali pri permeabiliziranih celicah, saj označena protitelesa lahko vstopijo v celice le, če je njihova membrana permeabilizirana.Type of material - undergraduate thesis ; adult, seriousPublication and manufacture - Ljubljana : [A. Lipovnik], 2019Language - slovenianCOBISS.SI-ID - 4783729
Author
Lipovnik, Anja
Other authors
Doljak, Bojan
Topics
Alzheimerjeva bolezen |
Citometrija |
pretočna citometrija |
sočasno zajemanje slike |
PC-12 |
fluorescenca |
gojenje celic
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Knjižnica UNK 0000000616 LIPOVNIK ANJA Vrednotenje 0000000182 IN: 0016292 Knjižnica UNK 616 LIPOVNIK ANJA Vrednotenje 182 IN: 0016292 |
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Database name | Field | Year |
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Lipovnik, Anja | ![]() |
Doljak, Bojan | 19785 |
Source: Personal bibliographies
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