Background: Increasing land demands for food production have led to biodiversity loss and land degradation in the Madhupur Sal forest. Reforestation activities such as agroforestry and woodlot ...plantation support the conservation of diversity, restoration of forest and prevention of soil erosion in degraded natural Sal forest. Knowing about these reforestation activities, this study is needed to compare the species composition, richness, and soil nutrients of these two plantation activities to the natural Sal forest in the degraded Madhupur Sal forest in Bangladesh. Results: The analysis showed that in between the reforestation activities, the highest Shannon-Wiener index (1.79), evenness (0.60) and Simpson's index (0.79) were found in the agroforestry site compared to the woodlot plantation site. On the contrary, the highest species richness (n = 14), tree basal area (19.56 m2 ha-1), Margalef's index (1.96) were recorded in woodlot plantation than in the agroforestry site. We observed that at 0-15 cm depth, soil organic matter (2.39%), total nitrogen (0.14%), available phosphorous (62.67 ㎍ g-1) and exchangeable potassium (0.36 meq/100 g) in agroforestry plots were significantly higher compared to other forest sites. At topsoil (15-30 cm depth), soil organic matter (1.67%) and available phosphorous (21.09 ㎍ g-1) were found to be higher in agroforestry site. Conclusions: Both reforestation approaches improved soil function, although woodlot plantation had the higher species richness. Therefore, plantation activities by the sustainable implementation of these two practices are the best alternative to restore the biodiversity, richness and conserve soil fertility in the Madhupur Sal forest of Bangladesh.
The global and national push to strengthen informal institutions’ role in increasing rural households’ access to justice has often met with skepticism in South Asia. This is because the impact of ...such initiatives on women’s welfare is debatable in many contexts due to reports of informal institutions’ hostile and oppressive behavior toward women. This study contributes to this debate by presenting the first empirical evidence of gender difference in trust in informal village institutions. The study also tests the relationship between a husband’s trust in informal institutions and his tendency to commit physical violence against his wife. It uses the Pakistan Rural Household Panel Survey datasets of more than 2,000 households from three provinces (Punjab, Sindh, and Khyber Pakhtunkhwa) of Pakistan. Trust in local political institutions is measured by (a) respondent’s reliance on village institutions for handling general community affairs and maintaining law and order and (b) respondent’s perceptions of local government’s effectiveness in dispute settlement and ensuring public security. Men’s and women’s trust in informal village institutions and their perceptions of these institutions’ legitimacy do not significantly differ in most cases. Women exhibit a greater trust and confidence in informal institutions that hold regular resident meetings than in those that do not. The results also reveal a significant negative relationship between a husband’s trust in informal institutions and the incidence of physical violence against his wife. Greater trust in informal institutions has a significant positive correlation with a husband’s psychological well-being, his relationship with family, and his perceptions of institutional legitimacy. The findings imply that well-performing informal institutions work as an indirect deterrent for domestic violence in the study areas of Pakistan.
This study examines population-weighted, sex- and age-disaggregated official COVID-19 mortality data (as of July 25, 2020) from the United States to understand gender gaps (men-women) across age. The ...analysis yields three key findings: (1) all age groups report about 8 percentage points more deaths among men than women; (2) non-elderly adults (<65 years) have a larger gender gap in reported mortality than elderly (≥65 years) adults; and (3) the gender gap in reported mortality varies across states, with thirteen states reporting more deaths among women than men. Women's lack of access to healthcare and a state's healthcare capacity have a significant correlation with the gender gap in reported mortality for both non-elderly and elderly adults. The findings underscore the possible presence of an underreporting bias against women in the officially reported COVID-19 death tolls in the US.
HIGHLIGHTS
The COVID-19 gender mortality gap in the US is similar to that of other developed nations.
While men are more likely than women to die from COVID-19 infections, the reverse is true in thirteen states.
The gap is high in states with low hospital capacity and women's low healthcare access.
COVID-19 fatalities among women are more likely to be underreported than those among men.
Tropical cyclones of the Bay of Bengal (BoB) that formed near the synoptic-scale dryline usually intensified over a distance of 600–800 km within 3 days and caused severe destruction after landfall. ...High-resolution simulations of very severe cyclonic storms in association with dryline indicate that the meridional shear aids in the development of a linear-shaped group of convective cells that mature as an east–west oriented quasi-linear convective system (QLCS) within the boundary between the dry-moist air masses. The leading edge deep convections are supported by low-level moist southwesterly inflow; however, the typical mid-level mesoscale convective vortex (MCV) associated with these QLCS is unremarkable due to a very narrow trailing stratiform region within the QLCS. Supercells are likely to be organized within the QLCS due to extremely unstable atmospheric conditions resulting from a strong vertical shear of 27–39 m s
−1
between 0 and 6 km and large convective available potential energy of > 3000 J kg
−1
. The vertical shear veering with height causes several numbers of low-level mesovortices having diameters less than 10 km at the leading edge in the different convective stages of the QLCS. The dryline aloft in the BoB produces horizontal positive shear vorticity of the order 10
–5
s
−1
with higher values in the levels 850–600 hPa. The advection of intense cloud-scale cyclonic mesovortices (~ 10
–3
s
−1
) assists and enhances a cyclonic vortex to the rear side of the QLCS that performs as an MCV for cyclogenesis over the BoB.
Duchenne muscular dystrophy (DMD) is a severe rare neuromuscular disorder caused by mutations in the X-linked dystrophin gene. Several mutations have been identified, yet the full mutational ...spectrum, and their phenotypic consequences, will require genotyping across different populations. To this end, we undertook the first detailed genotype and phenotype characterization of DMD in the Bangladeshi population. We investigated the rare mutational and phenotypic spectrum of the DMD gene in 36 DMD-suspected Bangladeshi participants using an economically affordable diagnostic strategy involving initial screening for exonic deletions in the DMD gene via multiplex PCR, followed by testing PCR-negative patients for mutations using whole exome sequencing. The deletion mapping identified two critical DMD gene hotspot regions (near proximal and distal ends, spanning exons 8-17 and exons 45-53, respectively) that comprised 95% (21/22) of the deletions for this population cohort. From our exome analysis, we detected two novel pathogenic hemizygous mutations in exons 21 and 42 of the DMD gene, and novel pathogenic recessive and loss of function variants in four additional genes: SGCD, DYSF, COL6A3, and DOK7. Our phenotypic analysis showed that DMD suspected participants presented diverse phenotypes according to the location of the mutation and which gene was impacted. Our study provides ethnicity specific new insights into both clinical and genetic aspects of DMD.
In Bangladesh, safely managed sanitation (SMS) coverage is low, and diarrheal disease is a significant health problem. This study estimated the inequality in access to SMS facilities at the national ...and sub-national levels and assessed the prevalence of diarrheal diseases in connection with these improved facilities. Data were extracted from the Bangladesh Demographic and Health Survey, conducted during 2017-2018. SMS was defined as using an improved sanitation facility, which designed to hygienically separate excreta from human contact and include the use of a flush toilet connected to piped sewer system, septic tank, ventilated improved pit latrine, pit latrine with a slab, and composting toilet. The slope index of inequality (SII) and multi-level regression models were used for inequality and risk factors of SMS respectively. The national coverage of SMS was 44.0% (45.3% and 43.5% in urban and rural areas, respectively). At the sub-national level, the lowest and highest coverage of SMS was observed in Mymensingh (32.9%) and Chittagong (54.1%) divisions, respectively. The national level SII indicated that wealthy households had access to higher SMS by 60.8 percentage points than poor households. Additionally, greater inequality was observed in rural areas, which was 71.9 percentage points higher in the richest households than in the poorest households. The coverage gap between the rich and poor was highest in the Sylhet division (85.3 percentage points higher in rich than in poor) and lowest in Dhaka (34.9 percentage points). Old and highly educated household heads and richest households had better access to higher levels of adequate sanitation. After adjusting for confounding variables, the prevalence of diarrheal disease was 14.0% lower in the SMS user group than in their counterparts. Substantial inequalities in access to SMS exist at both national and sub-national levels of Bangladesh, with the prevalence of diarrhea being lower among SMS users. These findings may help to prioritize resources for reducing inequality and expanding the coverage of improved sanitation in Bangladesh.
Background: Reproductive management practices play crucial roles to maximize the reproductive performance of cows, and thus contribute to farm profitability. We aimed to assess the reproductive ...management of cows currently practiced in the dairy farms in an urban farming system. Methods: A total of 62 dairy farms were randomly selected considering all size of farms such as small (1-5 cattle), medium (6-20 cattle) and large farms (> 20 cattle) from selected areas of Dhaka city in Bangladesh. The reproductive management-related parameters viz. estrus detection, breeding method, pregnancy diagnosis, dry cow and parturition management, vaccination and treatment of reproductive problems etc. were obtained in a pre-defined questionnaire during the farm visit. Results: The visual observation method was only used (100.0%; 62/62) for estrus detection irrespective of size of the farms; while farmers observed cows for estrus 4-5 times a day, but only for 20-60 seconds each time. Regardless of farm size, 89.0% (55/62) farms used artificial insemination (AI) for breeding the cows. Intriguingly, all farms (100.0%) routinely checked the cows for pregnancy at 35-40 days post-breeding using rectal palpation technique by registered veterinarian. However, only 6.5% (4/62) farms practiced dry cow management. Notably, all farms (100.0%) provided nutritional supplements (Vit D, Ca and P) during late gestation. However, proper hygiene and cleanliness during parturition was not practiced in 77.4% (48/62) farms; even though 96.7% (60/62) farms treated cows by registered veterinarian for parturition-related problems. Conclusions: While farmers used AI service for breeding and timely check their cows for pregnancy; however, they need to increase observation time (30 minutes/ observation, twice in a day: early morning and early night) for estrus detection, consider dry cow management and ensure hygienic parturition for maximizing production.
형질전환 벼에서 brazzein 감미단백질의 안정적인 발현 이예림(Ye Rim Lee); 샤히나(Shahina Akter); 이인혜(In Hye Lee) ...
Journal of plant biotechnology,
03/2018, Volume:
45, Issue:
1
Journal Article
Open access
Brazzein is the smallest sweet protein and was isolated from the fruit pulp of Pentadiplandra brazzeana Baillon, native to tropical Africa. From ancient times, the indigenous people used this fruit ...in their diet to add sweetness to their daily food. Brazzein is 500 to 2000 times sweeter than sucrose on a weight basis and 9500 times sweeter on a molar basis. This unique property has led to increasing interest in this protein. However, it is expensive and difficult to produce brazzein other than in its native growing conditions which limits its availability for use as a food additive. In this study, we report high production yields of, brazzein protein in transgenic rice plants. An ORF region encoding brazzein and driven by the $2{\times}CaMV\;35S$ promoter was introduced into rice genome (Oryza sativa Japonica) via Agrobacterium-mediated transformation. After transformation, 17 regenerated plant lines were obtained and these transgene-containing plants were confirmed by PCR analysis. In addition, the selected plant lines were analyzed by Taqman PCR and results showed that 9 T0 lines were found to have a single copy out of 17 transgenic plants. Moreover, high and genetically stable expression of brazzein was confirmed by western blot analysis. These results demonstrate that recombinant brazzein was efficiently expressed in transgenic rice plants, and that we have developed a new rice variety with a natural sweetener. Brazzein은 열대식물인 P. brazzeana Baillon의 과실에서 분리된 가장 작은 감미단백질로 토착민들의 단맛원료로 사용되어 왔다. Brazzein은 sucrose보다 분자량 기준으로 500 ~ 2000배, 몰 기준으로 9500배 당도가 높아 감미료로써 매우 높은 평가를 받고 있다. 그러나 이 감미단백질은 재배가 어렵고 생산 비용이 높아서 brazzein 단백질의 이용 가능성을 높이기 위한 대체 생산 시스템으로 형질전환 식물체 육성 하고자 하였다. 본 연구에서는 brazzein 관련 유전자를 벼에 도입하기 위하여 식물형질전환용 Ti-plasmid에 $2{\times}CaMV\;35S$ 프로모터에 의해 지배되어 발현하도록 하고, 선발 마커로 bar 유전자가 삽입된 식물발현 벡터를 구축하여 A. tumefaciens EHA105에 형질전환시켜 17개의 재분화 식물체를 육성하였다. 17개 재분화 식물체는 PCR 및 RT-PCR 분석을 통하여 유전자 도입 및 발현을 확인하였으며, TaqMan PCR을 통해 single copy로 도입된 T0 세대 9개체를 선발하였다. 또한 FST 분석을 통하여 도입 유전자가 intergenic으로 삽입된 개체 5개를 선발하였다. 이들 5개체를 이용하여 western blot 분석에 의해 단백질 발현량을 분석한 결과 선발된 모든 개체에서 발현 밴드를 확인하였다. 그 중 brazzein 단백질의 발현량이 높은 개체를 TG11으로 계통화하여 후대 종자를 육성하였다. TG11 계통은 천연 감미료 brazzein을 생산하는 새로운 벼 품종을 개발하기 위한 육종 소재로 활용 가능하다고 시사된다.
PDF
